FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104440
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT94830013.347990833901052Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT150400.21169859974888944Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT147500.20761664536543345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143340.20176115218088966Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT96940.13644988204559402Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT92520.13022842053701628Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC74610.10501883329298298RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC71640.10083834897613322RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT796600.0134.356482
ACTGCAA799300.0134.282361
CCGCAAT112850.0126.630632
TGCAATT948100.0125.815013
CGCAATT123550.0124.3453753
ACCGCAA121300.0117.719941
GCAATTT1294250.0104.8478554
AATTTCC1361100.0104.579856
CAATTTC1339500.0104.45315
ATTTCCA1388650.0102.111327
TTTCCAC1389850.0101.5321358
TTCCACA1430400.098.841359
CGGCAAT20450.076.9343262
GACCGGA36500.072.8998952
AGACCGG37050.072.374641
AGAACGG44150.058.5959431
ACGGAAG44600.057.8704154
GGCAATT30050.056.6976243
ACCGGAA46900.056.5782743
ATGCAAT126500.056.5692332
Produced by FastQC (version 0.11.5)