FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716053
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT290708624.812844393926863Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT480710.4103002948177172Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT448170.3825264361641246Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT431840.3685882950512429Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT299160.2553419654212899Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT282630.2412331183547906Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC225220.19223197436884246RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC220140.18789604314695402RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC172770.1474643380326122Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC119410.10191998960742155RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1193800.0133.263372
ACTGCAA1207400.0132.077061
TGCAATT1412450.0126.412963
CGCAATT182300.0123.807133
CCGCAAT175100.0119.5437242
ACCGCAA192600.0109.248231
GCAATTT1926150.0105.173194
AATTTCC2021650.0104.717436
CAATTTC1994800.0104.6082155
ATTTCCA2065100.0102.179797
TTTCCAC2077850.0101.167278
TTCCACA2137900.098.563029
GACCGGA85750.091.064212
AGACCGG90200.087.588121
AGAACGG96250.077.5517961
ACCGGAA101000.077.0967563
ACGGAAG95300.077.067234
GAACGGA98250.075.641042
AGATTGG110200.072.285491
GATTGGA110100.071.451042
Produced by FastQC (version 0.11.5)