FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15145357
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184347112.171855704688902Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT302350.19963213808693978Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT300090.1981399315975186Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT279570.18459122488826113Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT188910.124731295538296Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT184980.12213644089076275Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1697000.0133.6281
CTGCAAT1694600.0133.40832
TGCAATT2014700.0126.767653
CGCAATT255900.0126.274463
CCGCAAT240550.0124.266492
ACCGCAA252700.0118.0030441
GCAATTT2755800.0105.25494
AATTTCC2901250.0104.911536
CAATTTC2859450.0104.70155
ATTTCCA2962450.0102.356867
TTTCCAC2965600.0101.8434458
TTCCACA3056650.098.95929
CGGCAAT42800.077.4138262
AGACCGG76550.070.161061
GACCGGA77600.068.297022
ATGCAAT275750.065.468122
ACGGCAA58400.056.7477871
GGCAATT62200.056.532023
ATTGCAA128750.056.3352471
TATGCAA99950.055.7711331
Produced by FastQC (version 0.11.5)