FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9755233
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT9298209.531499657670913Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT277500.2844627083740593Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT203440.2085444806905176Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT188720.1934551435111801Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT163990.16810464701355674Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1025650.0129.268811
CTGCAAT1073200.0123.2654952
ACCGCAA132450.0113.133981
CCGCAAT134000.0112.15672
TGCAATT1333600.0109.5733643
CGCAATT154000.0104.792623
AATTTCC1819200.095.663786
GCAATTT1715800.095.602094
CAATTTC1772900.094.825265
TTTCCAC1850900.093.6327068
ATTTCCA1859200.093.362887
TTCCACA1925550.090.4131249
GACCGGA48050.075.139212
AGACCGG48400.074.544241
CGGCAAT29800.073.471922
GTTCCAC80750.060.9611935
ACCGGAA60400.060.0149273
AGATTGG86450.058.31041
GATTGGA88200.055.9769482
ACGGAAG53900.055.819454
Produced by FastQC (version 0.11.5)