FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7516093
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT167948422.3451732169892Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT489730.6515752266503355Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT363900.48416111934751205Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT332050.44178537971789333Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT293190.39008298593431456Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT137440.18286096247079434Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC117730.15663723160423906RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC117660.1565440981105476RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC114490.1523264813248053Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC89530.11911773843138983Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGACCTCCAGTCCATCTCGTATGCCGTCTT88880.11825292741854045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT84210.1120395929108381Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA730050.0128.227461
CTGCAAT759850.0123.004042
TGCAATT936000.0110.922243
CCGCAAT86050.0108.93682
GACCGGA59400.0105.573912
CGCAATT99350.0102.16013
AGACCGG64000.098.930611
ACCGCAA95850.097.566881
AATTTCC1287450.095.001766
GCAATTT1214050.094.791844
CAATTTC1254700.094.014655
TTTCCAC1306550.093.141318
ATTTCCA1312300.092.976287
TTCCACA1356950.089.9436349
AGATTGG94150.089.820861
ACCGGAA71000.088.426153
GATTGGA95600.087.665132
ATTGGAA106950.078.428473
CCGGAAG80050.078.3386154
AGAACGG65200.076.236781
Produced by FastQC (version 0.11.5)