EGAF00005483446 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005483446
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11985484
Sequences flagged as poor quality	0
Sequence length	151
%GC	47

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT	1689352	14.094983565119273	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT	25857	0.2157359686100286	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT	22200	0.18522405937048517	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT	18312	0.15278481870235694	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT	17208	0.1435736762904193	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CTGCAAT	69435	123.73507	2
TGCAATT	76535	121.00375	3
ACTGCAA	73935	116.38923	1
CGCAATT	9290	111.60568	3
GCAATTT	99180	105.130165	4
CCGCAAT	9495	104.849205	2
AATTTCC	105425	102.21238	6
CAATTTC	104450	101.462776	5
TTTCCAC	108325	99.28111	8
ATTTCCA	111750	96.47991	7
TTCCACA	111745	96.294464	9
ACCGCAA	10885	91.085014	1
GACCGGA	5200	72.22973	2
AGACCGG	5620	67.8028	1
AGAGCAC	516905	54.386276	9
CGGAAGA	522250	54.179195	5
ACCGGAA	7035	54.107967	3
GAAGAGC	524805	54.014698	7
AAGAGCA	523200	53.985497	8
TCGGAAG	519635	53.573494	4