FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8443595
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT121840614.429943643673104Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT186560.22094854146841483Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT162090.19196799467525386Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT128020.15161788314100808Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT125770.1489531414048163Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC94380.11177703336079003Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT595850.0129.464922
TGCAATT667850.0125.9878543
ACTGCAA621850.0124.386151
CGCAATT81650.0114.8166353
CCGCAAT79150.0110.7550662
GCAATTT875050.0107.8442154
AATTTCC920800.0105.906086
CAATTTC908850.0105.515485
TTTCCAC941950.0103.236268
ATTTCCA965350.0101.1557
TTCCACA969900.0100.4182369
ACCGCAA88550.099.333561
GACCGGA41100.067.745052
AGACCGG42600.065.4189451
CGGCAAT21250.059.030352
ACCGGAA53550.052.127123
CGGAAGA4271850.048.551585
AGAGCAC4202900.048.4582569
AAGAGCA4237300.048.307828
GAAGAGC4275600.048.2534987
Produced by FastQC (version 0.11.5)