FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10664507
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT146285613.717052274427688Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT370000.346945245570189Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT208420.19543331913983458Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT182510.17113777505139244Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT131910.12369066849503686Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT903450.0132.331152
ACTGCAA926000.0129.861911
TGCAATT1004050.0128.5433
CGCAATT119350.0119.0200653
CCGCAAT115000.0116.093222
GCAATTT1327000.0109.1357354
AATTTCC1403750.0107.374116
CAATTTC1378550.0106.557845
ACCGCAA127250.0104.766431
TTTCCAC1437700.0104.425068
ATTTCCA1447150.0103.96867
TTCCACA1486100.0101.302179
GACCGGA44750.071.1413962
CGGCAAT29600.069.823892
GATTGGA101850.067.071922
AGATTGG104300.065.787181
ATTGGAA105150.064.408883
AGACCGG49300.064.293781
ACCGGAA60300.052.6701933
TTGGAAG133000.051.0240944
Produced by FastQC (version 0.11.5)