FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13154277
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7576765.759921278835773Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT186010.14140647942870596Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT912150.0126.826762
TGCAATT995750.0124.27143
ACTGCAA938850.0123.772051
CGCAATT106000.0112.544983
GCAATTT1271100.0107.616034
CCGCAAT107950.0105.613432
AATTTCC1351200.0105.486746
CAATTTC1329750.0104.352165
TTTCCAC1388650.0102.250358
ATTTCCA1412850.0100.724747
ACCGCAA114400.099.7438351
TTCCACA1436250.099.018069
CGGCAAT31700.053.30722
GACCGGA38500.041.0662422
ATGCAAT142800.038.90362
ACGGCAA43700.038.345461
TCCGATC41950.037.6770365
AGACCGG43450.036.7296071
GATTGGA93900.035.8378222
AGATTGG95150.035.4509241
Produced by FastQC (version 0.11.5)