FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3315188
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1724895.202993012764283Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46850.1413192856634375Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA335300.0132.189681
CTGCAAT333700.0132.14752
TGCAATT381800.0126.10713
CGCAATT40550.0121.5977253
CCGCAAT39850.0117.009952
ACCGCAA41450.0112.338681
GCAATTT511500.0105.2586444
AATTTCC540250.0104.283956
CAATTTC528050.0103.85445
TTTCCAC544550.0102.8888
ATTTCCA555050.0101.359627
TTCCACA565300.099.316579
CGGCAAT11400.072.517062
ACGGCAA14200.057.717681
ATGCAAT46350.057.5755652
GTTCCAC26550.055.980635
GGCAATT16250.055.7781453
GCAAGTT26050.050.9521031
AAGTTCC28950.046.086813
GACCGGA12550.043.914712
Produced by FastQC (version 0.11.5)