FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11057371
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT352564631.885029452299285Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1055400.9544764302472983Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT804130.727234348924351Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT715900.6474414216543879Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT638120.5770992037799944Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT283170.2560916152673181Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC231290.20917268670826006RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC230500.20845823116543705RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC193960.17541240137461245Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGACCTCCAGTCCATCTCGTATGCCGTCTT187640.1696967570320287Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT176790.15988429799452328Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC176370.15950446087049083Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
NGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT138160.12494832632458475Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC131390.11882571363482332Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC123140.11136462726989986RNA PCR Primer, Index 16 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)