FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11561939
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT11157629.650301735720971Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT332450.2875382753705931Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT248950.2153185551316263Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT225780.19527866389884949Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT199340.17241052733455867Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA846850.0122.6784741
CTGCAAT854750.0121.506182
TGCAATT1023000.0111.5897453
CCGCAAT104700.0106.639192
CGCAATT113700.0104.188863
ACCGCAA109200.0102.318551
GCAATTT1296200.098.160054
AATTTCC1390650.097.7074666
CAATTTC1356250.096.363685
TTTCCAC1430450.094.597028
ATTTCCA1449350.093.613557
TTCCACA1494600.090.89099
GACCGGA57200.078.077792
AGACCGG58400.077.22621
ACCGGAA71850.062.7614973
CGGCAAT27200.060.5061872
AGATTGG104450.054.6000941
ACGGAAG61250.054.447934
GATTGGA104250.054.1061132
AGAACGG65800.051.0742381
Produced by FastQC (version 0.11.5)