FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13377396
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1580231.1812687611251098Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1481800.0132.978471
CTGCAAT1490950.0131.74232
ACCGCAA179800.0129.850391
TGCAATT1670900.0127.556613
CGCAATT194850.0124.420953
CCGCAAT187250.0124.04142
GCAATTT2153150.0111.441174
CAATTTC2217300.0109.027945
AATTTCC2276650.0108.876346
TTTCCAC2271100.0108.4043358
ATTTCCA2325750.0106.2528847
TTCCACA2325800.0105.939739
CGGCAAT39150.080.1674042
ACGGCAA51150.061.5318071
ATGCAAT218850.056.600952
GGCAATT77350.043.205123
GTTCCAC98650.041.0942765
TCCGATC36900.039.9681365
CGTAATT15200.039.6709183
CCGATCT41650.039.506216
Produced by FastQC (version 0.11.5)