FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4198433
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51021812.1525816894065Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143680.3422229198369963Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT89030.21205530730155753Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA65660.1563916823252866No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA53100.12647575893196344No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51290.12216462665951795Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT43430.10344335612834597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT786900.0139.293692
ACTGCAA790100.0139.16781
CCGCAAT99350.0132.816972
TGCAATT910200.0131.262133
ACCGCAA101000.0130.307311
CGCAATT110150.0128.4993
AATTTCC1275600.0109.7121356
GCAATTT1231650.0109.652174
CAATTTC1246950.0109.1283655
TTTCCAC1282550.0108.3081368
ATTTCCA1290300.0108.0789267
CGGCAAT17100.0107.930252
TTCCACA1325650.0104.9639
GTTCCAC34100.084.3793265
GGCAATT23250.083.1292653
ACGGCAA22200.082.5391
ATTGCAA38600.080.813071
GCAAGTT33200.080.597461
GTAATTT38250.074.084694
TGTAATT35650.072.3542563
Produced by FastQC (version 0.11.5)