FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12018563
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5836404.856154600179739Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT154180.12828488730308274Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1327500.0133.485262
ACTGCAA1339100.0132.824081
TGCAATT1512200.0128.508533
CCGCAAT178200.0126.363992
CGCAATT190400.0125.0951843
ACCGCAA181500.0124.689341
GCAATTT2019950.0109.0798344
AATTTCC2109350.0107.847086
CAATTTC2070400.0107.436095
TTTCCAC2129250.0106.174898
ATTTCCA2167100.0104.705117
TTCCACA2201050.0102.856279
CGGCAAT38050.077.3412
ATTGCAA99850.060.7946321
ACGGCAA48550.059.895971
GGCAATT52300.058.6285743
ATGCAAT176700.056.0902332
GTTCCAC84850.047.0987555
GCAAGTT87500.040.7943761
CATGCAA88550.039.8180541
Produced by FastQC (version 0.11.5)