FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14138429
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7298595.162235493066451Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT196370.1388909616478606Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1383250.0133.376022
ACTGCAA1402250.0131.855481
TGCAATT1557000.0128.400473
CGCAATT192400.0124.490583
CCGCAAT182600.0123.654662
ACCGCAA189800.0119.308341
GCAATTT2041450.0110.546714
AATTTCC2140150.0109.118366
CAATTTC2097150.0108.497825
TTTCCAC2158550.0107.2509168
ATTTCCA2208050.0105.348847
TTCCACA2228300.0104.0376369
CGGCAAT39550.071.638812
ACGGCAA52200.053.7558561
GGCAATT57750.050.822023
ATGCAAT187050.050.8019372
ATTGCAA103750.044.0728871
TATGCAA87500.044.032851
GTTCCAC91100.041.8667875
CGTAATT15950.040.081553
Produced by FastQC (version 0.11.5)