FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6330947
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT157886824.9388914486253Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT430700.6803089648357505Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT266490.42093228706542635Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT158460.2502943082606757Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT120100.18970305706239524Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT114350.18062068755274685Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC111910.1767666037956091RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC109960.17368649587494572RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA90370.14274325784120448No Hit
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC86160.13609338381761843Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA72530.11456421922344318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1091350.0139.1922
ACTGCAA1100650.0138.539251
TGCAATT1246150.0131.759543
CCGCAAT142200.0131.342712
CGCAATT154400.0127.922883
ACCGCAA157500.0118.837821
GCAATTT1664750.0111.4836654
AATTTCC1720550.0111.330226
CAATTTC1685600.0110.949345
TTTCCAC1728150.0109.968198
ATTTCCA1738000.0109.8120657
TTCCACA1780850.0106.791289
AGATTGG70600.099.455871
GATTGGA72450.097.060062
ATTGGAA76750.091.3345953
TTGGAAG78100.089.848844
CGGCAAT25150.088.097872
GGCAATT28800.082.226443
AGACCGG38700.080.0122761
GACCGGA38800.079.01042
Produced by FastQC (version 0.11.5)