FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4373170
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT53118612.14647498267847Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT205270.46938490843026914Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT189840.43410157848883074Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143120.32726832023452096Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT115590.2643162740071847Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT55820.12764196223791893Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT446300.0132.708372
ACTGCAA450550.0131.692171
TGCAATT504150.0127.389263
CGCAATT56550.0125.317573
CCGCAAT54400.0123.06282
GCAATTT630900.0114.594234
ACCGCAA58750.0114.234741
AATTTCC699500.0113.754826
CAATTTC672900.0113.372585
ATTTCCA719250.0110.150017
TTTCCAC720050.0109.64128
TTCCACA764750.0103.5739449
GACCGGA35650.0101.8369142
AGACCGG36500.098.70131
ACCGGAA41250.088.715883
CGGCAAT13300.079.160972
AGATTGG48750.076.432281
AGAACGG33050.075.819631
GATTGGA49500.075.103392
CCGGAAG50900.072.752374
Produced by FastQC (version 0.11.5)