FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912550
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT57065914.585347152113073Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT222180.5678649474128127Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT211070.5394691441642918Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156710.4005316225990722Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT127530.32595110605615263Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT56410.14417707121953713Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT43230.11049060075909573Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC41010.10481655186515189RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT40200.10274629078222643Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT286700.0128.886382
TGCAATT312200.0125.6635363
ACTGCAA303250.0121.500281
CGCAATT34600.0117.543753
CCGCAAT34350.0113.1135562
GCAATTT391100.0111.788294
AATTTCC437700.0111.043016
CAATTTC421700.0110.131975
GACCGGA34950.0109.71712
TTTCCAC452700.0106.496318
ATTTCCA454950.0106.379817
AGACCGG37900.0100.8196951
TTCCACA481800.0100.0349
ACCGCAA39450.097.963311
ACCGGAA39900.096.105593
AGATTGG48200.082.7416461
GATTGGA49350.081.087162
ACGGAAG35500.079.990064
AGAACGG36600.078.796741
CCGGAAG51650.074.382874
Produced by FastQC (version 0.11.5)