FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5718840
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4988178.722345790405047Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT192560.3367116408222647Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT180740.3160431136384302Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT135580.2370760503878409Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT109680.191787145644921Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT440100.0131.02682
TGCAATT484100.0129.180423
ACTGCAA464050.0124.102311
CGCAATT56800.0120.784263
GCAATTT600200.0117.062164
AATTTCC673850.0115.0417256
CAATTTC645350.0114.643235
CCGCAAT57500.0114.011012
ATTTCCA701250.0110.057087
TTTCCAC698650.0109.885768
ACCGCAA60650.0107.766341
TTCCACA736350.0104.624119
GACCGGA36350.090.8727952
AGACCGG36400.090.7781751
ACCGGAA44000.075.238323
AGATTGG54750.068.444261
GATTGGA58200.064.116092
CGGCAAT15950.063.267552
AGAACGG36200.062.3911931
ACGGAAG34900.061.9894454
Produced by FastQC (version 0.11.5)