FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5913451
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT63281110.70121321712144Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT244390.4132781348826599Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT226850.38361694381165923Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT171190.28949254842899685Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT137430.23240236538697961Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT65790.11125483241511598Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT532400.0132.617142
TGCAATT590600.0129.94943
ACTGCAA554750.0127.018531
CGCAATT70700.0123.240833
CCGCAAT68200.0119.773552
GCAATTT752300.0114.9608464
AATTTCC831350.0114.748726
CAATTTC796700.0114.465045
ATTTCCA858150.0110.634937
TTTCCAC859700.0109.736368
ACCGCAA75650.0108.581751
TTCCACA910150.0103.804979
AGACCGG42550.093.025091
GACCGGA42750.092.9060442
ACCGGAA49850.079.9650043
AGATTGG59750.075.3627851
GATTGGA60200.074.297972
CGGCAAT17000.072.609382
GTTCCAC53550.072.267965
ACGGAAG43800.071.780524
Produced by FastQC (version 0.11.5)