FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3443150
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT35936010.436954532913175Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT140350.40762092851023046Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT124810.36248783817144187Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT93850.27257017556597885Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79720.23153217257453207Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT406850.0133.864872
ACTGCAA407000.0133.50341
TGCAATT461100.0129.718573
CCGCAAT49650.0128.836352
ACCGCAA51900.0122.8723751
CGCAATT55850.0122.592523
GCAATTT584050.0115.0415954
AATTTCC644900.0114.7367256
CAATTTC619700.0114.362055
ATTTCCA659450.0111.440357
TTTCCAC659400.0110.933948
TTCCACA692550.0105.749579
AGACCGG23850.099.58331
GACCGGA24050.099.024022
GTTCCAC32050.086.310985
ACCGGAA27950.084.4261553
CGGCAAT10950.077.5806662
ACGGAAG24950.072.169984
GATTGGA37850.070.017852
CCGGAAG33950.069.719344
Produced by FastQC (version 0.11.5)