FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10826071
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6853326.330385233941288Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT262200.24219312805171886Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT238460.22026458167510632Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT180560.16678257513736977Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT150910.1393949845701178Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT688900.0124.78262
TGCAATT743650.0124.167163
ACTGCAA733350.0116.857291
CGCAATT90000.0116.165323
GCAATTT920900.0113.0484
AATTTCC1034400.0111.192826
CAATTTC996700.0110.263015
CCGCAAT91500.0109.817992
TTTCCAC1068800.0106.5193948
ATTTCCA1085850.0105.3648767
ACCGCAA97500.0102.792061
TTCCACA1131400.0100.856429
GACCGGA53950.082.0912552
AGACCGG56050.078.649911
ACCGGAA68750.064.4189453
CGGCAAT28250.058.3399282
ACGGAAG61850.057.284434
AGAACGG62850.055.2341581
AGATTGG97150.049.936581
GATTGGA97750.049.764152
Produced by FastQC (version 0.11.5)