FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4314422
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT101209823.45848412603125Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT393740.9126135551876937Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT359330.8328577964788795Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT268550.6224472246803859Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT224110.5194438559788542Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT99980.231734401502681Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71860.16655765245031665Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71340.16535239251051473Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC70650.16375310528270068RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC68770.15939562703880147RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC56230.13033032002896333Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC49390.11447651620541523Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46620.10805618921839358Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT486850.0135.344482
ACTGCAA496250.0132.829351
TGCAATT552300.0129.469023
CGCAATT69200.0122.1472853
CCGCAAT65450.0121.268352
GCAATTT708900.0114.57454
AATTTCC783650.0114.343756
CAATTTC750450.0114.170885
GACCGGA57250.0113.776852
AGACCGG58200.0112.946691
ATTTCCA800200.0111.356487
TTTCCAC808650.0109.789278
AGATTGG66000.0109.613311
ACCGCAA72400.0109.454621
GATTGGA68450.0104.814282
TTCCACA854350.0103.9429
ACCGGAA62950.0103.243863
ATTGGAA71450.0101.226463
TTGGAAG74400.096.724764
CGGCAAT15900.090.4284442
Produced by FastQC (version 0.11.5)