FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989943
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3332048.351096744991093Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129470.3244908511224346Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT120390.3017336337887534Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT91530.22940177340879303Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72550.18183217153728762Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT401700.0132.891532
TGCAATT446250.0129.709753
ACTGCAA412050.0129.490051
CGCAATT53650.0124.8996053
CCGCAAT50150.0122.615962
ACCGCAA51100.0120.233571
GCAATTT568550.0114.794244
AATTTCC627150.0114.1814656
CAATTTC603600.0113.4325265
TTTCCAC646400.0109.946278
ATTTCCA653550.0109.101987
TTCCACA681150.0104.347839
GACCGGA24150.096.1982962
AGACCGG24600.095.650271
ACCGGAA30400.076.8973543
CGGCAAT12750.075.73142
GTTCCAC39500.072.782535
AGAACGG25850.066.583111
GAACGGA25900.065.872282
ACGGAAG27250.062.3416674
Produced by FastQC (version 0.11.5)