FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14154129
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8419005.948087656965681Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT231660.1636695553643746Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT142870.1009387437404308Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT750900.0122.7087252
TGCAATT824700.0121.4078753
ACTGCAA776700.0119.1564251
CGCAATT102100.0114.710033
GCAATTT1069350.0105.645844
CCGCAAT106950.0103.328062
AATTTCC1152550.0101.669616
ACCGCAA109450.0101.222591
CAATTTC1129500.0101.075825
TTTCCAC1181750.098.690598
ATTTCCA1221750.095.762387
TTCCACA1233950.094.70959
CGGCAAT33950.044.2996562
GATTGGA101450.041.5380172
AGATTGG107000.039.40511
ATGCAAT127250.039.2826232
ATTGGAA113550.037.4949343
ACGGCAA45950.032.4322131
GGCAATT49850.032.0641443
TCCGATC48950.031.4671615
Produced by FastQC (version 0.11.5)