FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10828952
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT260310224.03835569683936Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT997240.9209016717407188Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT914970.8449294077580176Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT690560.6376979046541161Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT568540.5250184874769045Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT269940.24927619958053188Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC206850.19101571417067875Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT190250.17568643761649327Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC187190.17286067940831207RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT186430.17215885710824094Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC180740.16690442436165567RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC148190.1368461140099245Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC132920.12274502648086352Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT126050.11640092226837832Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT607550.0125.047642
TGCAATT675900.0122.189923
ACTGCAA664500.0114.073021
GACCGGA141850.0113.6492162
AGACCGG144650.0111.033941
GCAATTT834250.0110.6348954
AATTTCC925700.0110.0230566
CAATTTC891000.0109.406485
CGCAATT79800.0108.198643
TTTCCAC956200.0105.402378
ATTTCCA967250.0104.792347
ACCGGAA157500.0102.8636253
AGATTGG172850.0100.777891
GATTGGA175600.098.6708152
TTCCACA1023000.098.5410469
CCGCAAT82650.097.965872
ACGGAAG140600.091.7793354
ATTGGAA190200.090.7531053
CCGGAAG178500.090.192474
AGAACGG145300.089.18971
Produced by FastQC (version 0.11.5)