FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168602
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT31033314.3102791568024Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT125030.5765465493437708Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT117540.5420081693182982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT87480.40339352264730916Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT70670.3258781463818626Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT31550.1454854325505556Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT25100.11574276884370668Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT22590.10416849195933602Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT206750.0132.130752
ACTGCAA209500.0129.849241
TGCAATT230550.0128.598463
CCGCAAT26250.0120.57922
CGCAATT27450.0119.2749943
GCAATTT291400.0114.4502264
AATTTCC323800.0113.3444756
CAATTTC311800.0113.080355
GACCGGA19350.0112.55262
ACCGCAA28250.0110.793551
TTTCCAC333050.0109.267678
ATTTCCA334200.0109.262577
TTCCACA352000.0103.385219
AGACCGG21050.0102.4613341
ACCGGAA22250.097.230273
GATTGGA27600.083.380672
ACGGAAG20500.083.220284
AGATTGG28250.081.745591
CCGGAAG26400.080.571034
AGAACGG21350.079.592781
Produced by FastQC (version 0.11.5)