FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4116537
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2265535.50348508953035Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT88100.21401483820016678Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79310.19266193890641575Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT60670.1473811604268345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51460.1250079860815049Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT312150.0129.079352
TGCAATT341150.0126.067083
ACTGCAA324100.0124.1888661
CGCAATT39350.0119.758213
CCGCAAT38650.0118.1697852
GCAATTT424700.0113.918134
AATTTCC477850.0112.200096
CAATTTC459350.0111.153885
ACCGCAA42050.0108.119371
TTTCCAC492950.0107.900048
ATTTCCA498300.0107.2291957
TTCCACA520650.0102.368399
GACCGGA20950.083.182612
AGACCGG22050.079.378631
CGGCAAT11900.070.7809452
ACCGGAA26100.067.0473863
GTTCCAC34800.055.4998555
ACGGCAA15400.055.1772841
ACGGAAG23050.051.0328034
AGAACGG22900.050.109121
Produced by FastQC (version 0.11.5)