FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162763
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT56667810.976254381616975Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT219390.4249468743771504Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT207310.4015485506501073Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT158730.30745164943655173Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT125140.2423895886756762Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT56120.10870148406967355Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT402700.0129.141142
TGCAATT451750.0126.581393
ACTGCAA417750.0124.574671
CGCAATT54350.0121.0601653
CCGCAAT53200.0116.715112
GCAATTT576550.0112.3570864
AATTTCC644850.0111.286516
CAATTTC620550.0110.870645
ACCGCAA58200.0107.0914841
TTTCCAC667300.0106.735588
ATTTCCA673400.0106.211077
TTCCACA707350.0100.856259
GACCGGA37350.098.969142
AGACCGG37500.097.6314851
ACCGGAA45300.081.440073
CGGCAAT14000.079.885012
AGATTGG54650.071.113861
AGAACGG38950.068.632681
GATTGGA57050.067.97642
ACGGAAG38900.067.955474
Produced by FastQC (version 0.11.5)