FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2740459
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT48937417.8573735275733Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT188160.6866003103859609Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT175600.6407685719800953Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134270.4899544200442335Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT106380.38818314742165455Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51600.1882896259349255Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA43280.157929748264798No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA37400.1364734885652367No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC35800.13063505055175062RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT35240.1285915972470305Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC35050.12789828273292905RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT34930.1274603998819176Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT30640.1118060879582581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA539950.0139.158571
CTGCAAT542400.0138.958822
CCGCAAT67300.0134.016052
TGCAATT615350.0132.799483
CGCAATT73300.0131.2693
ACCGCAA71350.0126.050241
AATTTCC868000.0117.0387656
CAATTTC833350.0116.812595
GCAATTT794400.0116.168464
ATTTCCA878700.0115.139197
TTTCCAC891250.0112.92518
GTTCCAC39650.0112.2709965
CGGCAAT11900.0106.792652
TTCCACA944850.0106.580429
GCAAGTT31100.0104.882491
CCAATTT26700.0104.712794
GACCGGA28550.0103.0146942
AAGTTCC32300.0102.296123
AGACCGG29650.099.2311
AGTTCCA34150.097.8176964
Produced by FastQC (version 0.11.5)