FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074754
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT147575529.080325864071437Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT569931.123069216754152Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT516211.0172118687920637Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT394790.7779490395002397Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT325360.6411345259297297Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156500.3083893327637163Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC112710.22209943575590066RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT107420.21167528514682682Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC106970.21078854265645192RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT104570.20605924937445244Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC88070.17354535806070598Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCCCACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT71920.14172115535058447Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC58670.11561151535621234RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC55160.1086949239312881TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCGCGTATGCCGTCTT54090.10658644734306334Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC53140.10471443541893853TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT340450.0130.220632
TGCAATT385050.0125.6060263
ACTGCAA360100.0123.26061
GACCGGA78300.0117.804142
AGACCGG79600.0115.557441
AGATTGG93900.0113.3572541
GCAATTT487150.0112.609594
GATTGGA95550.0111.815132
AATTTCC543100.0111.5973056
CAATTTC524050.0111.111185
ACCGGAA83200.0110.9534453
CGCAATT51000.0110.369773
TTTCCAC558150.0107.577938
ATTTCCA562400.0107.1468667
CCGCAAT51200.0104.548092
CCGGAAG89850.0102.822374
AGAACGG68600.0102.418791
TTCCACA595600.0100.9840859
ACGGAAG70650.0100.130274
ATTGGAA107500.099.11523
Produced by FastQC (version 0.11.5)