FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8487796
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133929015.779007883789856Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT503120.592756941849215Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT468740.5522517270678985Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT353710.41672773473820535Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT291840.3438348424019616Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133260.157001888358297Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98070.11554236223396509Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT95960.1130564400935178Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC94110.1108768401125569RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC93320.109946092012579RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT501350.0124.850162
TGCAATT545450.0122.1428153
ACTGCAA547650.0114.012461
CGCAATT65250.0112.261993
GCAATTT665500.0112.010814
AATTTCC751200.0109.249986
CAATTTC721050.0108.888815
GACCGGA79250.0108.827672
TTTCCAC769150.0105.537078
AGACCGG83150.0104.803781
CCGCAAT66800.0104.223172
ATTTCCA789450.0103.4125447
TTCCACA814400.099.877959
ACCGGAA89800.095.718853
ACCGCAA79050.087.640421
AGATTGG105650.086.195781
ACGGAAG73250.084.242924
AGAACGG74250.083.623361
GATTGGA110250.083.034512
CCGGAAG106250.080.011074
Produced by FastQC (version 0.11.5)