FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4109079
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT47646611.595445110692689Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT127000.30907169222105485Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT80020.19473950245298277Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT46770.11382112633999004Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT417750.0134.550372
ACTGCAA425350.0132.646851
TGCAATT490950.0127.5804443
CGCAATT57300.0122.993123
CCGCAAT54650.0120.4544752
ACCGCAA58350.0113.142721
GCAATTT644550.0108.922874
AATTTCC672800.0108.148296
CAATTTC661250.0107.1279145
TTTCCAC680100.0106.166788
ATTTCCA691100.0105.032867
TTCCACA702200.0103.176429
ATTGCAA33600.071.193161
CGGCAAT11800.069.5795062
AGATTGG30600.063.916431
GATTGGA31800.062.3764152
ATTGGAA32650.060.527043
GGCAATT14950.058.8041343
ATGCAAT56100.056.080252
GACCGGA16550.051.804672
Produced by FastQC (version 0.11.5)