FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140913
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2913687.036322666040073Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT114840.27733014434256403Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT105750.25537846363833294Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT79680.19242133316976232Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT65500.15817767724170972Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT307550.0130.496612
TGCAATT338800.0125.897173
ACTGCAA318950.0125.7111
CGCAATT34400.0114.199513
GCAATTT422100.0111.9070364
AATTTCC477900.0110.336276
CAATTTC455700.0109.8212665
CCGCAAT34450.0107.7102662
TTTCCAC494950.0105.89768
ATTTCCA500000.0105.003697
ACCGCAA36800.0100.862531
TTCCACA527700.099.3803569
GACCGGA23750.081.9401252
AGACCGG24000.081.413771
ACCGGAA29050.068.2404563
CGGCAAT10950.063.662342
GATTGGA34600.063.170722
GTTCCAC37700.061.4425935
AGATTGG37800.057.2639471
AGAACGG23100.054.7134441
Produced by FastQC (version 0.11.5)