FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9113466
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2338822.566334257460334Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT703700.0130.730992
ACTGCAA722300.0127.953951
TGCAATT777300.0126.803333
CGCAATT95200.0121.966363
ACCGCAA92450.0119.222311
CCGCAAT93700.0117.8821642
GCAATTT994600.0111.409984
CAATTTC1028700.0108.813085
AATTTCC1050450.0108.505646
TTTCCAC1062700.0106.554698
ATTTCCA1094250.0103.872997
TTCCACA1094600.0103.699689
CGGCAAT21600.056.5941432
ACGGCAA27850.044.1773951
TCCGATC36450.042.3138435
ATTGCAA57100.041.3092651
TTCCGAT36850.040.8673974
ATGCAAT101150.039.3493352
CCGATCT43700.036.4695056
GGCAATT40650.033.825593
Produced by FastQC (version 0.11.5)