FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11903021
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6685535.6166665588509Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143240.12033919792294746Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1038900.0132.987172
ACTGCAA1070100.0129.777331
TGCAATT1160100.0128.58763
CGCAATT141350.0126.399733
CCGCAAT140350.0121.980632
ACCGCAA145650.0117.948331
GCAATTT1488650.0112.698454
AATTTCC1560550.0110.7958766
CAATTTC1538150.0110.263475
TTTCCAC1581150.0108.647768
ATTTCCA1610200.0107.016027
TTCCACA1623250.0105.983439
CGGCAAT29100.065.7573242
ATGCAAT151300.050.3968932
ACGGCAA39150.048.7146841
GACCGGA38450.042.954722
AGACCGG39050.042.501491
TCCGATC54700.040.961615
TTCCGAT54600.040.237214
GGCAATT49750.039.4806483
Produced by FastQC (version 0.11.5)