FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8253731
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8065919.772441093609666Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT313290.379573795172147Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT282930.3427904301703072Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT213450.25861031817004937Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT177020.21447270331441623Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT85380.10344412726801976Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT639550.0130.086612
TGCAATT716450.0126.6353453
ACTGCAA675600.0123.157331
CGCAATT82850.0118.423233
GCAATTT898050.0113.310744
AATTTCC1000700.0112.276746
CAATTTC963400.0111.428945
CCGCAAT83500.0110.6304552
ATTTCCA1040250.0107.4808657
TTTCCAC1038100.0107.285728
ACCGCAA89450.0103.381011
TTCCACA1097050.0101.818179
GACCGGA55450.092.4649662
AGACCGG57300.088.996971
ACCGGAA65650.077.2137763
ACGGAAG56800.071.216124
AGAACGG59400.069.585471
CGGCAAT21850.069.4654542
AGATTGG78600.068.576311
GATTGGA80550.066.807742
Produced by FastQC (version 0.11.5)