FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10217753
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144822614.173625062183437Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT553090.5413029655345946Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT513390.5024490218152661Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT390150.3818354191963732Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT315400.3086784344855469Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT152050.14880962575626952Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC118900.11636609340625087Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT110890.10852679644927804Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC104770.10253722124619767RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT682050.0125.30542
TGCAATT753000.0122.3013463
ACTGCAA715950.0118.825441
CGCAATT86700.0114.803233
GCAATTT946000.0109.452334
GACCGGA86950.0109.379882
CCGCAAT87400.0109.065922
AATTTCC1066700.0107.305586
CAATTTC1024200.0106.843085
AGACCGG91550.0103.920171
TTTCCAC1093900.0103.752448
ATTTCCA1108250.0102.8478557
TTCCACA1170350.097.012269
ACCGCAA101450.093.4927751
ACCGGAA104200.092.3174743
ACGGAAG86450.081.879534
AGATTGG127950.080.202671
CCGGAAG120400.079.896024
AGAACGG87400.079.854411
GATTGGA129650.078.89952
Produced by FastQC (version 0.11.5)