FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4709664
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT66339214.085760682715371Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT256450.5445186747929364Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT236910.5030295154813591Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT178790.37962368440721034Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144310.306412516901418Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT56360.11966883412489722Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACCCGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT51000.10828797977944923Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT47420.10068658825767612Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT347700.0129.266312
TGCAATT382500.0126.216053
ACTGCAA358450.0125.032431
CGCAATT44000.0116.304233
GCAATTT477300.0113.496134
AATTTCC535950.0112.104976
CAATTTC516150.0110.733385
ATTTCCA556000.0107.525617
TTTCCAC554300.0107.330768
CCGCAAT46150.0105.380972
AGACCGG39800.0104.1785661
GACCGGA39900.0103.877692
TTCCACA587950.0101.274249
ACCGCAA48950.099.687791
ACCGGAA46900.088.528333
AGATTGG56100.081.157751
GATTGGA56750.080.325392
ACGGAAG39900.079.6820144
AGAACGG39600.078.849441
GAACGGA41800.076.06012
Produced by FastQC (version 0.11.5)