FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7403010
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1665262.249436377905744Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT698300.0131.2362
ACTGCAA702350.0130.415561
TGCAATT793650.0127.590563
ACCGCAA93600.0123.916211
CGCAATT102500.0122.259813
CCGCAAT95650.0121.906642
GCAATTT1000050.0115.291044
AATTTCC1112750.0113.670226
CAATTTC1067100.0113.5693745
TTTCCAC1139850.0109.809238
ATTTCCA1158900.0108.557587
TTCCACA1202050.0104.50729
CGGCAAT23800.076.26582
GTTCCAC70450.061.6280145
ACGGCAA30450.058.912671
ATGCAAT110900.054.5356182
GACCGGA23500.052.8317572
GCAAGTT65350.049.6776431
CCAATTT64750.049.0013924
AGACCGG25650.048.1349561
Produced by FastQC (version 0.11.5)