FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6122029
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4382017.157773999437114Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT173230.2829617435657361Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156400.2554708577826077Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT117780.1923871971204318Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT97820.15978362729088672Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT600500.0131.448292
ACTGCAA607400.0129.35741
TGCAATT678400.0127.293873
CGCAATT82700.0123.018683
ACCGCAA76300.0121.474581
CCGCAAT77700.0120.7479862
GCAATTT854900.0114.7568054
AATTTCC955050.0113.7440646
CAATTTC912700.0113.630745
ATTTCCA985550.0109.682667
TTTCCAC980450.0109.586528
TTCCACA1036200.0103.683529
GACCGGA32400.092.3401642
AGACCGG34550.085.987341
CGGCAAT20500.084.306592
GTTCCAC57050.069.4979255
ACCGGAA43300.069.2628863
ACGGCAA26950.064.147161
CCAATTT50100.060.151824
ACGGAAG34150.059.5395744
Produced by FastQC (version 0.11.5)