FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5887966
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT975041.6559878232992515Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT687200.0136.655032
ACTGCAA696000.0135.454151
TGCAATT780050.0129.34613
ACCGCAA88450.0129.181261
CGCAATT92950.0128.887923
CCGCAAT89200.0127.878442
GCAATTT1042100.0108.881824
AATTTCC1087950.0107.313536
CAATTTC1070000.0107.25285
TTTCCAC1099700.0105.3965158
ATTTCCA1112650.0104.612517
TTCCACA1123750.0103.383199
CGGCAAT16800.075.345292
ACGGCAA21250.060.9619331
ATTGCAA48700.055.4424741
GGCAATT26550.051.5064163
ATGCAAT85300.048.6971132
TATGCAA33600.042.4535981
TCCGATC18950.042.2232325
CCGATCT22150.037.937046
Produced by FastQC (version 0.11.5)