FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252856
Sequences flagged as poor quality0
Sequence length151
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4173577.945334880682052Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT86730.16511018006204622Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA64600.12298071753727878No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT57000.10851239782701068Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT56700.10794127994371062Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA55460.10558065935940372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA774150.0139.288971
CTGCAAT778600.0137.88882
TGCAATT883950.0130.628363
CCGCAAT100300.0130.416952
CGCAATT106050.0128.74563
ACCGCAA102200.0128.699481
GCAATTT1172800.0110.581344
CAATTTC1191150.0110.238565
AATTTCC1210850.0110.0356756
TTTCCAC1222500.0108.2531368
ATTTCCA1230700.0107.938977
TTCCACA1249350.0106.087969
CGGCAAT15150.093.161052
ACGGCAA20400.069.221511
ATTGCAA39100.069.0663761
GGCAATT20700.068.8754653
AGACCGG18150.061.7609021
GACCGGA18750.058.9776652
TCGGTTA2150.057.5160834
ATGCAAT83350.054.8149832
Produced by FastQC (version 0.11.5)