FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005483636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17345440
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT310567117.904826859393594Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT655070.3776612181645435Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT423500.2441563892296765Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT414060.2387140366574731Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT379110.2185646486915293Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC330580.19058611369904713Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC286240.16502319918087982RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC285320.16449280041324982RNA PCR Primer, Index 16 (96% over 25bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT257510.14845976810043449Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC219260.12640786281581787Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)