FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10384212
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT505211448.65187652178133Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1071591.0319415666783383Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT683900.6585959531642844Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT677120.652066810654482Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT622630.5995929204835186Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC473590.45606734531228754RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC462250.44514692111447646RNA PCR Primer, Index 16 (96% over 25bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT413090.39780582291655836Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC359200.34590973296770133Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC281480.2710653441975183Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC258100.24855039554277206RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC226730.21834107393030883TruSeq Adapter, Index 16 (95% over 22bp)
AGATNGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT198900.19154077362827338Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC187470.18053367939714635TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC167610.16140849204542435TruSeq Adapter, Index 16 (95% over 22bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA148700.14319815504537078No Hit
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT143450.13814240310193976Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC141400.1361682523430762Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT132620.12771310909291914Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA122910.11836237549849714No Hit
AGATCGGAAGGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC114110.10988797224093652RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTACAGTCCATCTCGTATGCCGTCTT113000.10881904183003968Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC111080.10697008111929918Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTGCAGTCCATCTCGTATGCCGTCTT107110.10314696964969514Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)