FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11227372
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2612642.327027197459922Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT796850.0129.96812
TGCAATT856050.0128.92683
ACTGCAA819350.0126.8349841
CGCAATT100900.0125.518323
CCGCAAT107450.0113.481082
GCAATTT1096250.0112.615294
ACCGCAA110000.0110.706931
AATTTCC1153500.0110.059546
CAATTTC1135250.0110.040925
TTTCCAC1185400.0106.212088
ATTTCCA1200550.0105.4027257
TTCCACA1220300.0103.518299
ATGCAAT94050.045.5630042
CGGCAAT26350.045.2813262
TCCGATC45100.042.259495
TTCCGAT48000.038.493914
CCGATCT56750.036.38036
ACGGCAA34850.034.462971
GGCAATT39100.032.000093
ATTGCAA68350.031.0968131
Produced by FastQC (version 0.11.5)