FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15381149
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4174932.7143160761266922Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1046300.0128.692122
TGCAATT1136000.0126.6788563
ACTGCAA1075400.0125.67921
CGCAATT139200.0123.6754463
CCGCAAT147200.0113.0645752
ACCGCAA148050.0112.6198041
GCAATTT1478800.0109.845314
AATTTCC1559650.0107.253456
CAATTTC1539950.0106.696575
TTTCCAC1603800.0103.6982968
ATTTCCA1621350.0102.928787
TTCCACA1651200.0100.794439
CGGCAAT37650.055.6423952
TCCGATC63100.047.4886135
TTCCGAT66350.044.724314
ACGGCAA49150.043.2371861
CCGATCT78150.039.4890176
GGCAATT57600.037.7548873
ATGCAAT149550.036.674422
CGATCTA30050.034.230467
Produced by FastQC (version 0.11.5)