FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833954
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT48581912.67148745133614Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103320.26948680135442415Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA83370.2174517482473707No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA68900.1797100330363901No Hit
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67380.17574545756156698Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT67300.17553679569447103Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT57790.15073211624343955No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT57110.14895849037312392Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCATTT51650.1347173179438251No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAGA48420.12629259505982596No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAG48160.12561444399176414No Hit
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC45250.1180243685761488RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC44980.11732013477469996RNA PCR Primer, Index 16 (96% over 25bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGGAAA44930.11718972110776497No Hit
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT41520.10829550902279995Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCTTTT40920.1067305450195803No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAG40120.10464392634862078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1017250.0142.75451
CTGCAAT1027150.0141.046222
CCGCAAT122150.0138.095232
ACCGCAA123950.0136.6861
TGCAATT1166300.0133.917723
CGCAATT135100.0132.151143
CGGCAAT18200.0117.902242
CAATTTC1587200.0111.568615
GCAATTT1570500.0111.551034
AATTTCC1615550.0111.362296
ATTTCCA1623900.0110.495517
TTTCCAC1619350.0110.4481058
TTCCACA1649350.0108.5066159
ACGGCAA21250.0100.345121
GGCAATT23100.096.649833
ATTGCAA39100.094.738961
TATGCAA28800.088.6956251
GTTCCAC25200.085.998425
GCAAGTT24050.080.492261
GACCGGA16500.076.266462
Produced by FastQC (version 0.11.5)