FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11716803
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3308322.8235688523567393Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT836500.0127.131622
ACTGCAA855150.0124.9995351
TGCAATT923000.0124.199723
CGCAATT113250.0120.613523
CCGCAAT111600.0116.7436452
ACCGCAA111600.0116.598061
GCAATTT1191500.0108.304644
AATTTCC1256600.0105.270736
CAATTTC1237950.0105.257325
TTTCCAC1282300.0102.495038
TTCCACA1310350.0100.3821959
ATTTCCA1314500.0100.260287
CGGCAAT26250.056.255972
TCCGATC45850.049.8114175
TTCCGAT47100.049.1071554
CCGATCT53150.046.069916
CGATCTA23150.039.425837
ATGCAAT133650.037.7739452
ACGGCAA40000.037.2976881
CTTCCGA65700.035.2046743
Produced by FastQC (version 0.11.5)